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KMID : 0363620100310020091
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Journal of Korean Oriental Medicine
2010 Volume.31 No. 2 p.91 ~ p.108
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Gene expression microarray analysis of Paeoniae radix on IL--stimulated primary human gingival fibroblast
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Kim Kyung-Ho
Chio Yeong-Gon
Hong Yeon-Mi
Yeo Su-Jung
Choi Ji-Hoon
Kim Young-Hong
Lee Je-Hyun
Lim Sabina
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Abstract
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Background & Objective: The aim of this study was to investigate the effect of P. radix on the inflammatory related gene expression in IL-1β-stimulated primary human gingival fibroblast using Whole Transcript Sense Target (WT-ST).
Method: Human gingival fibroblast was incubated with P. radix [100 or 200 ㎍/㎖], and IL-1β [1ng/㎖] added an hour later. After 24h, total RNA was extracted using RNeasy Mini Kit and the whole gene expression patterns were performed using WT-ST Labeling Assayⓡ.
Result: In the DEG results, 782 genes were up-regulated in the IL-1β-treated group as compared to control and among those, 43 genes were associated with inflammation. 981 genes were down-regulated after treatment with IL-1β and of those 7 genes were associated with inflammation. 1439 genes were up-regulated after treatment with P. radix plus IL-1β-treated when compared to IL-1β-treated alone group and 1225 genes were down-regulated in the same condition. Among the down-regulated genes, 5 were associated with inflammation- and inhibitor genes such as GDF15 and LIF. In the analysis of the P. radix plus IL-1β-treated group, the most significant pathways were the cytokine-cytokine receptor interaction, toll-like receptor signaling, JAK-STAT signaling and tyrosine metabolism. The gene expression patterns in the P. radix 200㎍/㎖ plus IL-1β-treated group appear to be more involved in the metabolism-related pathways than in the 100㎍/㎖ plus IL-1β-treated group.
Conclusion & Discussion: By microarray analysis of gene expression data, we are able to identify gene expression patterns associated with not only anti-inflammation effect but also transcription function of P. radix.
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KEYWORD
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Paeoniae radix, human gingival fibroblast, microarray, IL-1β, anti-inflammation, cytokine
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